Avoidance of reporter assay distortions from fused dual reporters
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چکیده
منابع مشابه
Obelin mutants as reporters in bioluminescent dual-analyte binding assay
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BACKGROUND The dual-luciferase assay has been widely used in cell lines to determine rapidly but accurately the activity of a given promoter. Although this strategy has proved very useful, it does not allow the promoter and gene function to be analyzed in the context of the whole organism. RESULTS Here, we present a rapid and sensitive assay based on the classical dual-luciferase reporter tec...
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ژورنال
عنوان ژورنال: RNA
سال: 2017
ISSN: 1355-8382,1469-9001
DOI: 10.1261/rna.061051.117